Pre-pro B cells (Hardy Fr. A)

Marker Expression

CD127 (IL-7RA)
Expression: +
Required
Lineage dump
Expression: -
Analysis strategy: Mouse CD3ε, TCRβ, TCRγδ, B220, CD19, Ly6C, Ter119, CD11c, CD11b, GR-1, NK1.1, CD8α
Required
CD19
Expression: -
Suggested
CD93 (AA4.1)
Expression: +
Clone: AA4.1
Suggested
Ly6D
Expression: +
Citations:
- [1]
Suggested
PDCA-1 (CD317)
Expression: -
Other notes: Required to eliminate contaminating plasmacytoid dendritic cells
Citations:
- [1]
Suggested
CD24
Expression: -
Clone: 30-F1
CD43
Expression: +

Early B cell that has undergone B-lineage specification but not B-lineage commitment.